Efficient delivery of abciximab using mesoporous silica nanoparticles: in-vitro assessment for targeted and improved antithrombotic activity
Abciximab (ABX) is a chimeric monoclonal antibody reported for antithrombotic activity but their delivery remains challenging due to its poor stability in a biological system. The purpose of this research was to deliver ABX on the target efficiently using mesoporous silica nanoparticles (MSN). ABX coated mesoporous silica nanoparticles (MSN-ABX) were formulated and analyzed for particle size, shape, zeta-potential, surface morphology and surface chemistry. XPS analysis confirmed the presence of ABX on the surface of amino functionalized mesoporous silica nanoparticles (MSN-NH2). The degree of ABX attachment was 67.53±5.81% which was demonstrated by the Bradford assay.
Highlights
- A novel delivery system of abciximab coated mesoporous silica nanoparticles (MSN-ABX) were fabricated
- MSN-ABX exhibits a significant ABX loading required for therapeutic activity and sustained release profile
- The in-vitro assessment and imaging studies confirms the considerable affinity of MSN-ABX towards activated platelets
- The mesoporous silica nanoparticle shows great potential for efficient delivery of abciximab
Furthermore, the targeting efficiency of the targeted nanoparticles has been evaluated by capturing the fluorescent images in-vitro which showed the significant accumulation of the ABX coated nanoparticles towards activated platelets. The significant (P<0.05) increase in affinity of DiD dye loaded nanoparticles towards the activated platelets was confirmed by using an in-vitro imaging through photon imager optima. The hemolysis study of the nanoparticle formulations revealed that they were non-hemolytic for healthy human blood. The in-vitro antithrombotic effects of MSN-ABX were observed by blood clot assay which revealed its superior antithrombotic activity over clinical injection of ABX and could be a promising carrier for improved ABX targeted delivery.
Vishnu Priya, Vikas, Abhishesh Kumar Mehata, Dharmendra Jain, Sanjeev Kumar Singh, Madaswamy S. Muthu,
Efficient delivery of abciximab using mesoporous silica nanoparticles: in-vitro assessment for targeted and improved antithrombotic activity, Colloids and Surfaces B: Biointerfaces, 2022, 112697, ISSN 0927-7765,
https://doi.org/10.1016/j.colsurfb.2022.112697.