Encapsulation of cannabidiol in oil-in-water nanoemulsions and nanoemulsion-filled hydrogels: a structure and biological assessment study

Abstract

Hypothesis

Lipophilic cannabidiol can be solubilized in oil-in water nanoemulsions, which can then be impregnated into chitosan hydrogels forming another colloidal system that will facilitate cannabidioil’s release. The delivery from both systems was compared, alongside structural and biological studies, to clarify the effect of the two carrier’s structure on the release and toxicity of the systems.

Experiments

Oil-in-water nanoemulsions (NEs) and the respective nanoemulsion-filled chitosan hydrogels (NE/HGs) were formulated as carriers of cannabidiol (CBD). Size, polydispersity and stability of the NEs were evaluated and then membrane dynamics, shape and structure of both systems were investigated with EPR spin probing, SAXS and microscopy. Biocompatibility of the colloidal delivery systems was evaluated through cytotoxicity tests over normal human skin fibroblasts. An ex vivo permeation protocol using porcine ear skin was implemented to assess the release of CBD and its penetration through the skin.

Findings

Incorporation of the NEs in chitosan hydrogels does not significantly affect their structural properties as evidenced through SAXS, EPR and confocal microscopy. These findings indicate the successful development of a novel nanocarrier that preserves the NE structure with the CBD remaining encapsulated in the oil core while providing new rheological properties advantageous over NEs. Moreover, NE/HGs proved to be more efficient as a carrier for the release of CBD. Cell viability assessment revealed high biocompatibility of the proposed colloids.

Excipients mentioned in the research: Tween 80, Labrasol, Maisine CC

Sotiria Demisli, Eleni Galani, Maria Goulielmaki, Fotios Kyrilis, Tanja Ilić, Farzad Hamdi, Milkica Crevar, Panagiotis L. Kastritis, Vasiliki Pletsa, Frédéric Nallet, Snežana Savić, Aristotelis Xenakis, Vassiliki Papadimitriou, Encapsulation of cannabidiol in oil-in-water nanoemulsions and nanoemulsion-filled hydrogels: a structure and biological assessment study, Journal of Colloid and Interface Science, 2022, ISSN 0021-9797,
https://doi.org/10.1016/j.jcis.2022.12.036.