A starch-based implant as a controlled drug release system: Non-invasive in vivo characterization using multispectral fluorescence imaging.

Solid implants are parenteral depot systems that can provide a controlled release of drugs in the desired body area over a few days to months. Finding an alternative for the two most commonly used polymers in the production of parenteral depot systems, namely Poly-(lactic acid) (PLA) and Poly-(lactide-co-glycolide) (PLGA), is of great importance due to their certain drawbacks. Our previous study showed the general suitability of starch-based implants for controlled drug release system. In this study, the system is further characterized and the release kinetics are investigated in vitro and in vivo by fluorescence imaging (FI). ICG and DiR, two fluorescent dyes with different hydrophobicity serving as a model for hydrophilic and hydrophobic drugs, have been used. In addition to 2D FI, 3D reconstructions of the starch implant were also used to assess the release kinetics in 3D mode. The in vitro and in vivo studies showed a fast release of ICG and a sustained release of DiR over 30 days from the starch-based implant. No treatment-related adverse effects were observed in mice. Our results indicate the promising potential of the biodegradable biocompatible starch-based implant for the controlled release of hydrophobic drugs.

2.1. Materials

The fluorescent dye 1,10-Dioctadecyl-3,3,30,30-Tetramethylindotricarbocyanine Iodide (DiR), was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). The starch (MAIZE STARCH AMYLO N-400) was kindly provided by Roquette (Lestrem, France). Testing media was Phosphate Buffered Saline (PBS) (Ph. Eur.) plus 1% sodium dodecyl sulfate (SDS), adjusted to pH 7.4. SDS was purchased from Sigma Aldrich Chemie GmbH (Munich, Germany). Indocyanine green (ICG) was purchased from Carl Roth GmbH, Karlsruhe, Germany.

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Golbarg Esfahani, Henrike Lucas, Frank Syrowatka, Karsten Mäder, A starch-based implant as a controlled drug release system: Non-invasive in vivo characterization using multispectral fluorescence imaging., Journal of Controlled Release, Volume 358, 2023, Pages 358-367, ISSN 0168-3659,
https://doi.org/10.1016/j.jconrel.2023.05.006.