Bacteriophage entrapped chitosan microgel for biofilm-mediated poly bacterial infection in burn wounds
Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) bacteria are most commonly present in burn wound infections. Multidrug resistance (MDR) and biofilm formation make it difficult to treat these infections. Bacteriophages (BPs) are proven as an effective therapy against MDR as well as biofilm-associated wound infections. In the present work, a naturally inspired bacteriophage cocktail loaded chitosan microparticles-laden topical gel is developed for the effective treatment of these infections. Bacteriophages against MDR S. aureus (BPSAФ1) and P. aeruginosa (BPPAФ1) were isolated and loaded separately and in combination into the chitosan microparticles (BPSAФ1-CHMPs, BPPAФ1-CHMPs, and MBP-CHMPs), which were later incorporated into the SEPINEO™ P 600 gel (BPSAФ1-CHMPs-gel, BPPAФ1-CHMPs-gel, and MBP-CHMPs-gel).
Highlights
- This research presents new perspectives on the use of the combination of bacteriophages to treat MDR bacterial infections.
- The chitosan microparticles loaded gel was able to maintain the biological activity of the entrapped phages and provide sustain release delivery.
- The developed formulation demonstrated excellent antibacterial and biofilm eradication efficacy.
- Non-invasive evaluation of burn wound healing in a pre-clinical rat model using ultrasound/photoacoustic imaging.
BPs were characterized for their morphology, lytic activity, burst size, and hemocompatibility, and found to be in Caudoviricetes class. Furthermore, BPSAФ1-CHMPs, BPPAФ1-CHMPs, and MBP-CHMPs had an average particle size of 1.19 ± 0.11, 1.42 ± 0.21, and 2.84 ± 0.28 μm, respectively, and expressed in vitro antibiofilm eradication potency. The ultrasound and photoacoustic imaging in infected burn wounds demonstrated improved wound healing reduced inflammation and increased oxygen saturation. The obtained results suggested that the incorporation of the BPs in the MP-gel protected the BPs, sustained the BPs release, and improved the antibacterial activity.
Read more here
Deepa Dehari, Dulla Naveen Kumar, Aiswarya Chaudhuri, Akshay Kumar, Rajesh Kumar, Dinesh Kumar, Sanjay Singh, Gopal Nath, Ashish Kumar Agrawal, Bacteriophage entrapped chitosan microgel for biofilm-mediated poly bacterial infection in burn wounds, International Journal of Biological Macromolecules, 2023, 127247, ISSN 0141-8130, https://doi.org/10.1016/j.ijbiomac.2023.127247.
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