Microencapsulation by a Spray Drying Approach to Produce Innovative Probiotics-Based Products Extending the Shelf-Life in Non-Refrigerated Conditions
Recently, there has been a growing interest in producing functional foods containing encapsulated probiotic bacteria due to their positive effects on human health. According to their perceived health benefits, probiotics have been incorporated into a range of dairy products, but the current major challenge is to market new, multicomponent probiotic foods and supplements. Nevertheless, only a few products containing encapsulated probiotic cells can be found as non-refrigerated products. In this work, spray drying technology was investigated in order to produce an innovative nutraceutical formulation based on lactic acid bacteria (LAB), and was able to ensure a good storage stability of probiotics (no less than 109 CFU/cps) in non-refrigerated conditions.
Probiotic-loaded microparticles from spray drying experiments were produced under different conditions and compared by thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and the enumeration of the number of viable cells in order to identify the formulation exhibiting the most promising characteristics. Results from the dissolution test revealed that the optimized formulation provides a suitable amount of living cells after digestion of microparticles stored for 12 months at room temperature and confirmed that the microencapsulation process by spray drying ensures a good protection of probiotics for nutraceutical purposes.
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Materials and Methods
Eudragit L30 D-55 (Methacrylic Acid–Ethyl Acrylate Copolymer 1:1) was supplied from Evonik Industries (AG, Darmstadt, Germany) as a 30% aqueous dispersion soluble above pH 5.5. Triethyl citrate, supplied from Merk Millipore (Darmstadt, Germany), was used as plasticizer to be added to Eudragit dispersions. Dissolution tests were performed with hydrocloric acid (HCl at pH 2) and phosphate buffer solution (PBS) at a pH value of 7.5 according to Pharmacopeia 8.0. Tween 80 (Sigma, Milan, Italy) was used as a non-ionic detergent for coating removal. All other reagents were analytical grade and Milli-Q water (Millipore, Molsheim, France) was used throughout.
Gullifa, G.; Risoluti, R.; Mazzoni, C.; Barone, L.; Papa, E.; Battistini, A.; Martin Fraguas, R.; Materazzi, S. Microencapsulation by a Spray Drying Approach to Produce Innovative Probiotics-Based Products Extending the Shelf-Life in Non-Refrigerated Conditions. Molecules 2023, 28, 860. https://doi.org/10.3390/molecules28020860
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