Hayashibara Maltose PH - For Culture Media

Hayashibara ― A world leading supplier of pharmaceutical grade Maltose PH

Founded as a starch syrup manufacturer in 1883, Hayashibara has grown into a company with strong research and development expertise, engaging in original and creative research. Utilizing our technological background in biotechnology and in-depth knowledge of saccharide production, Hayashibara became the first company in the world to succeed in the mass production of “Trehalose”, a unique disaccharide recognized as a beneficial excipient for the biopharmaceutical industry. In addition to trehalose, Hayashibara has been supplying for many years other high purity ingredients to the pharmaceutical industry worldwide.

Maltose PH – For Culture Media

What is MALTOSE PH?

Maltose is a reducing disaccharide consisting of two glucose molecules linked by an α-1,4 bond
Maltose is manufactured from starch by enzyme technology
Maltose PH is highly purified crystalline maltose monohydrate with low endotoxin

Effect of Maltose PH supplementation on antibody production by CHO cell

Methods

CHO-K1 cells were inoculated at 0.3 × 106 cells/mL in duplicates in a DMEM/F12-based protein free chemically defined medium (PFCDM) supplemented with 2 g/L D-(+)-glucose, or glucose with an additional 10 g/L MALTOSE PH in single-use Erlenmeyer flasks.
The cultures were incubated in a humidified incubator at 37℃, 8% CO2 and a rotation speed of 110 rpm. Cell culture supernatants were collected daily on days 0-5 and 7, and monoclonal IgG antibody (anti-Her2) titers were determined by nephelometry using IMMAGE 800 (Beckman Coulter).

Maltose PH - For Culture Media_result — Maltose PH – For Culture Media_result

Two g/L of glucose was chosen as the base glucose concentration because this will allow for a premature but controlled cell growth constraint due to glucose depletion on Day 4.
IgG titer reached a maximum of 340 mg/L on Day 5 in the culture containing 2 g/L glucose as a carbon source.
IgG production continued in the MALTOSE PH supplemented cultures Days 5 through 7 to reach 600 mg/L.

Effect of Maltose supplementation on cell viability and viable density

Methods

The CHO-K1 cells were inoculated and cultured as described in the previous experiment.
Viable cell density (VCD) and culture viability were analyzed by Vi-Cell XR Cell Viability Analyzer (Beckman Coulter, Brea, CA) according to manufacturer’s instructions.

Summary

MALTOSE PH can be used by the CHO-K1 cells as a carbon source to maintain culture viability and IgG production upon glucose depletion.
MALTOSE PH supplementation to the CHO-K1 cell culture in addition to glucose increased the IgG titer compared to the culture with only glucose as a carbon source.
MALTOSE PH supplementation along with glucose improved the growth of CHO-K1 cell compared to the culture without MALTOSE PH supplementation.

Product Information – HIGH PURITY MALTOSE

Maltose PH - For Culture Media_high purity maltose — Maltose PH – For Culture Media_high purity maltose

See the full brochure on “Hayashibara Maltose PH – culture media brochure” here

(click the picture to download the brochure)

Source: Hayashibara brochure “Hayashibara Maltose PH – culture media brochure”

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