Preparation of Melatonin Nanoparticles and their Enhancement of Lungs Bioavailability in the Treatment of Lungs Cancer

Background: To develop a first-time-novel polymeric NPs from PLGA and their coating by Chitosan (CS) of Melatonin (MT) for the enhancement of lungs bioavailability by targeting lungs from the nose.

Materials and Methods: The Melatonin LC-MS/MS method was developed for the first time for a comparative Pulmokinetic evaluation administered by different routes which will be useful for the treatment of lung cancer and in vitro evaluation on lung cancer cell-lines (H1299). The solvent evaporation method was used to develop the PLGA nanoparticles and their encapsulation by CS.

Results: CS MT PLGA MPs were given a significantly high release and permeation of MT as compared to MT PLGA NPs. Different routes of administration were used to compare the pulmokinetics parameters. Lung cancer cell lines (H1299) were used for anticancer activities. PDI, ZP, PS, DL, and EE of CS MT PLGA NPs were observed at 0.131±0.009, +19.5±1.37mV, 98.36±7.15 nm, 4.19±0.38%, and 74.16 ± 5.29% respectively. Mucoadhesive nature of CS MT PLGA NPs was showed greater than MT PLGA NPs and MT S with 0.303 retention time with mas’ spectra of 233.20/174.10. The developed method was linear (1-1000ng/mL) with inter and intra-day accuracy (91.68-98.95%) followed by precision (1.67—2.69%). C_max and AUC_0-24 were significantly enhanced (p<0.001) than i.v. and oral in the lungs.

Conclusion: Capping of CS on PLGA NPs could be a strong possible nanocarrier for Melatonin to enhance their solubility of a drug (MT), entrapment, sustain and controlled release, and stability, with their therapeutic application.

Materials

We have purchased Melatonin from AK Scientific, USA; PLGA and chitosan from Tokyo Chemical Industry Co. Ltd., Japan. Most of the chemicals like DCM and other analytical solvents (Methanol, Ethanol, Acetonitrile, etc) are from Sigma-Aldrich, USA. Milli-Q-Water was used for all types of preparations and analysis.

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J YOUNG PHARM. VOL 15/ISSUE 2/2023 ORIGINAL ARTICLE, Preparation of Melatonin Nanoparticles and their Enhancement of Lungs Bioavailability in the Treatment of Lungs Cancer, April 17, 2023, Niyaz Ahmad1,2, Mohammed Saifuddin Khalid3, Ayman Makki Al Ramadhan1, Mohammed Riyad Al Hammad1, Mohd Faiyaz Khan4, Ziyad Saeed Almalki4, Faisal K Alkholifi5, Zabih Ullah6, Mohd Ibrahim Khan7 and Nazia Khan8, Received 19 October 2022; Revised 27 November 2022; Accepted 17 December 2022, Published in: J Young Pharm, ; 2023; 15(2): 314-325; Published online: 17 April 2023, DOI: 10.5530/jyp.2023.15.42

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